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Click to edit Master title style
Click to edit Master text styles
Second level
Third level
Fourth level
Fifth level
May 201= 0
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------=_NextPart_01CD2131.17AC3BD0 Content-Location: file:///C:/8909BD13/2012tissuesamples_files/pres.xml Content-Transfer-Encoding: quoted-printable Content-Type: text/xml; charset="utf-8" ------=_NextPart_01CD2131.17AC3BD0 Content-Location: file:///C:/8909BD13/2012tissuesamples_files/slide0049.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" Collecting a Plant Sample
3DLOGONEW
Updated April 2012
Collecting P= lant Tissue Samples
= NCDA&CS Agronomic Division
Plant/Waste/Solution/Media Section
3DXmasTree3
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3DLOGONEW
Plant Tissue Analysis — Why?
lTo fine-tune fertilization by monit= oring plant-nutrient uptake
lTo detect nutritional problems (such= as “hidden hunger”) prior to visual manifestation
lTo diagnose suspected nutrient probl= ems
lTo optimize ti= ming of harvest (tobacco)<= span style=3D'mso-special-format:lastCR;display:none'>
3DSoyKDef1
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3DLOGONEW
When to Sample (in general)
Predictive (routine) samples =
lPrior to or during early reproductive (bloom/flower) stage . . . for most crops =
3D"Tissue
lWeekly or biweekly  . . . for intensively managed crops    (e.g., cotton, greenhouse & plasticulture crops, turfgrass)
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3DLOGONEW
When to Sample (in general)
Diagnostic (problem) samples
lWhenever you suspect that a plant growth problem (slow growth, poor color) is related to nutrition=
3DCott01S 3Dxmas10
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3DLOGONEW
When to Sample (specifically)
lThe correct time to sample varies by crop.
l
lCrop-specific sampling recommendations are available in Appendix A of the NCDA&CS Plant = Tissue Analysis Guide, which is available online = at
www.ncagr.gov/agronomi/pdffi= les/plantguide.pdf
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3DLOGONEW
Plant Part to Sample
<= span style=3D'font-size:86%'>lDepends on crop and stage of growth
<= span style=3D'font-size:86%'>l<= span style=3D'font-size:86%'>Is usually= the Most Recent Mature Leaf (MRML)
&#= 8211;<= b>horticultural crops, like blackberry, muscadine grape, pepper, sweetpotat= o, Irish potato, trellis and greenhouse tomato, cucumber & strawberry
<= span style=3D'font-size:83%;color:#006666;mso-color-index:3;text-shadow:auto'><= span style=3D'mso-special-format:bullet;color:#003366;mso-color-index:1;positio= n: absolute;left:-4.32%;top:.3em;font-size:75%'>–<= b>agronomic crops, like soybean & peanut&= #13;
–
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3DLOGONEW
What is the MRML?
lThe first, full-sized and hardened-off leaf . . . not soft and succulent like a newly emerged leaf <= /span>
lUsually the 3<= /span>rd=  to 5= th=  leaf= back from the growing point=
lNote<= b>: Some crops—like soyb= ean, strawberry & tomat= o—have compound leaves with many leaflets. The MRML includes all the leaflets.
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3DLOGONEW
Selecting the MRML
Tomato MRML =
5th=  ent= ire leaf from the plant&#= 8217;s growing point
Illustration by K. Tomlinso= n
1=
2=
3=
4=
5=
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3DLOGONEW
Other Tissue: MRML + Petiole
lThis is the required sample for cotton & strawberry.
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file:///C:/8909BD13/2012tissuesamples_files/slide0055.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" Collecting a Plant Sample
3DLOGONEW
Other Tissue: Petioles Only
l<= span style=3D'font-size:86%'>For wine grapes (Vitis vinifera), petioles of MRMLs opposite fruit clusters are the recommended = sample. Sampled shoots should be well exposed (generally the 1st or 2nd fruit cluster from the b= ase of the shoot).
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3DLOGONEW
Other Tissue: Top of Plant
l<= span style=3D'font-size:86%'>This is the appropriate sample for forage crops like bermuda or fescue.
3Dtwofists
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